Recombinant protein expression and purifification
Technical service advantages
More than 10 years of experience inrecombinant protein research
High success rate of expression
Convenient separation operation
5L-500L large-scale expression capacity
One-stop protein customization
High purity, high bio-activity
Characteristics of eukaryotic expression cells
It has the function of post-translational modi-fication and folding, which can make theexpressedprotein closer to natural molecularstructure, physicochemical properties andbiological functions.
Characteristics of prokaryotic expression cells
It has a clear genetic background, low cost, highexpression amount, and relatively simple separa-tion and purification process.
Prokaryotic cell‒based protein expression
Service Content
Plasmid construction:Target fragment insertion,Plasmid sequencing
Period:1-2weeks
Specifific deliverable:Sequencing report
Service Content
Expression for small scale:Obtain the strain containing the plasmid of interest,Expression detection
Period:1week
Specifific deliverable:Feasibility report
Service Content
Expression for large-scale:Protein purifification,SDS-PAGE/HPLC analysis
Period:2-4weeks
Specifific deliverable:If the expression method is feasible and thereis still some remaining sample after QC testing,0.1-0.5mg sample can be provided
Service Content
Formulation and lyophilization
Period:1week
Specifific deliverable:
- Purifified proteins
- SDS-PAGE report
- COA
HEK293 cell‒based protein expression
Service Content
Gene synthesis and codon optimization (optional)
Plasmid construction:Target fragment insertion,Plasmid sequencing,Plasmids extracted for large-scale
Period:1-2 weeks
Specifific deliverable:Sequencing report
Service Content
Protein expression and purification:Transfection of HEK293 cells transiently,Proteinpurifification
Period:2-3weeks
Specifific deliverable:Feasibility report
Service Content
Purity detection :SDS-PAGE/HPLC analysis
Period:1week
Specifific deliverable:If the expression method is feasible and thereis still some remaining sample after QC testing,0.1-0.5mg sample can be provided
Service Content
Expression for large-scale(optional)
Period:2-3weeks
Formulation and lyophilization
Period:1week
Specifific deliverable:
- Purifified proteins
- SDS-PAGE report
- COA
CHO stable cell‒based protein expression
Service Content
Gene synthesis and codon optimization (optional)
Plasmid construction:Target fragment insertion,Plasmid sequencing,Plasmids extracted for large-scale
Period:1-2weeks
Specifific deliverable:Sequencing report
Service Content
Stabilized cell line construction, protein expression, and purification:stable transfection,pool selection,High-expression monoclonal cell line selection,Tertiary cell banking (optional)
Period:10-14weeks
Specifific deliverable:Expression report2-3 stable cell lines (optional)
Service Content
Purity detection:SDS-PAGE/HPLC analysis
Period:1week
Specifific deliverable:If the expression method is feasible and thereis still some remaining sample after QC testing,0.1-0.5mg sample can be provided
Service Content
Expression for large-scale(optional)
Period:4-5weeks
Formulation and lyophilization
Period:1week
Specifific deliverable:
- Purifified proteins
- SDS-PAGE report
- COA
