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rhuman LBP

文字:[大][中][小] 2018-3-15  浏览次数:81

Characteristic

The LBP is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

huLBP-content

0.25 mg/ml phosphate-buffered saline, pH 7.2 (determined by huLBP-ELISA with defined standard)
For reconstitution add 40 µl water/ tube

Purification

By his-tag with metal affinity purification with Talon and controlled by SDS page (purity:90-95%).
Attention: His-tag has no protease site and can’t be splitted off

Endotoxin content

Less than 0.01ng/ml (determined by LAL-ELISA-test „Chromogenix“ after heating 5' 75°C)

Special note

Up to 0.2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (FACS).

Quantity

10 µg, lyophilized

Storage

long time: -80°C. Short time storage is possible at 4°C or -20°C

 

Attention!

The LBP will be precipitated by rapid changes of pH or temperature.
Best working temperature is ice bath.

 

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